![]() Both single cell 5' and V(D)J enriched libraries can be combined and sequenced using a 2x150 cycle sequencing profile. V(D)J enriched libraries are sequenced with a 2x150 cycle sequencing profile. An asymetrical sequencing profile is used to sequence stand alone 10X single cell 5' libraries. ![]() Single cell libraries prepared using the 10X Chromium Controller are Illumina compatable and will be sequenced using the NovaSeq 6000. The power of Single Cell 5' expression combined with V(D)J enrichment immune profiling can provide significant understanding of the adaptive immune system in the following research areas: Simultaneously measure cell surface protein expression with gene expression (using the Feature Barcoding application - inquire).Link full length, paired TCR α and β chain sequences with TCR-pMHC specificity.Simultaneously measure TCR, B cell Ig, cell surface protein expression and 5' gene expression in the same cells.Pair heavy and light chain immunoglobulin (Ig) sequences from individual B cells for full isotype resolution.Pair α and β chain TCR sequences from individual T cells.Assemble and annotate full length V(D)J gene sequences.Reveal clonality, diversity, antigen specificity and cellular context.The 10X Genomics workflow allows your project to reveal insights into T and B cell diversity, V(D)J recombination and immune cell profiling. Combined with the optional V(D)J enrichment for immune profiling of human or mouse T or B cells, a comprehensive cellular context of the adaptive immune response and immune repertoires of ≤80,000 human or mouse T or B cells can be determined on a cell-by-cell basis per 10X Chromium run. From translational immunology and immuno-oncology, to infectious disease research-these technological advancements, along with our intuitive software analysis and visualization tools, will accelerate the understanding of the adaptive immune system.The Chromium Single Cell 5' Gene Expression solution allows the individual cell characterization of 500 to 10,000 cells per sample and 80,000+ cells per run.A comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of T and B cells on a cell-by-cell basis.The Chromium Single cell Immune Profiling Solution Encapsulate up to 80,000 cells in less than 10 minutes.More efficient than leading academic droplet systems.Complete practical solution for single cell analysis Identify rare cell types in heterogeneous populations.High-throughput single cell transcriptomic measurements enable discovery of gene expression dynamics for profiling individual cell types.Key advantages of the Chromium Single cell RNA-Seq Finally, the Chromium™ Software Suite is utilized for processing, analysis and visualization of single cell gene expression data. ![]() High-quality next generation sequencing libraries are finished in single bulk reaction. ![]() Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells are performed inside each GEM. Single cells, reagents and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-sized GEMs (Gel Bead in emulsion) using the GemCode Technology. The Chromium™ Single Cell RNA sequencing Solution The GemCode™ Technology from 10X Genomics, which combines microfluidics with molecular barcoding and custom bioinformatics software enable analyzing the transcriptomes of thousands of cells at single cell resolution. Advances in single cell RNA quantification techniques have enabled comprehensive study of cell subpopulations within a heterogeneous population. ![]()
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